LeClaire Institute

Module 4 · Final Review

The whole curriculum on one chart, then five whole foods read label line by line: plain yogurt, canned beans, rolled oats, olive oil and a simple bread. Each label line is matched to its branch and lesson, and the key molecules behind it are shown in ball-and-stick form. The labels use typical values for illustration; they are not any brand’s label. The figure is on the left and the lesson script on the right; the mixed quiz at the end covers every module.

Previous lesson: Module 3 · Flavorings

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Figure 1

The Whole Curriculum Family Tree

Every lesson on one chart, grouped the way a label is read.

What the tree shows
Two branches are on the Nutrition Facts panelMacronutrients and micronutrients are the nutrients the panel counts: grams of fat, carbohydrate and protein, and milligrams or micrograms of a few vitamins and minerals.
Part of the third branch is in the ingredient listAdditives, processing words and flavorings show up in the ingredient list, and sometimes in words like “cultured”, “enriched” or “pasteurized”.
The rest of the third branch is never printedAntinutrients and bioactives, such as phytate and polyphenols, come with the whole food but have no label line. You find them by knowing the food, which is what the next five figures practise.
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Figure 2

Reading a Label Line by Line

Which branch each line of the Nutrition Facts panel and ingredient list belongs to.

The Nutrition Facts panel
Label lineBranchLesson
Caloriesenergy from protein and carbohydrate (4 kcal per gram), fat (9) and alcohol (7)Fats, Fig. 1 · Water and Ethanol, Fig. 5
Total Fat, Saturated Fat, Trans Fatfats: triglycerides grouped by their fatty acid chainsFats, Fig. 4
Cholesterola sterol, not a fat; listed in milligramsOther Lipids, Fig. 3
Sodiuma major mineral; salt is about 2.5 times the sodium figureMajor Minerals, Fig. 8
Total Carbohydrate, Dietary Fiber, Total Sugars, Added Sugarscarbohydrates: starch, fiber and sugarsCarbohydrates, Fig. 8
Proteinchains of amino acidsProtein, Fig. 7
Vitamin D, Calcium, Iron, Potassiumthe four micronutrients every US label must listFat-Soluble Vitamins, Fig. 7 · Major Minerals, Fig. 8 · Trace Minerals, Fig. 8
% Daily Valuehow much of a day’s reference amount one serving gives; 5% or less is low, 20% or more is high—
The ingredient list
Order by weightIngredients are listed from most to least by weight, so the first one or two make up most of the food.
Additives and flavor linesAdditives appear by name or, in the EU, by E-number, and flavorings as “natural flavor” or “artificial flavor” (Additives; Flavorings).
Processing words“Cultured”, “pasteurized”, “enriched”, “rolled” and “hydrogenated” each name a process from the Processing lesson.
What is not printedAntinutrients and bioactives have no line. A single-ingredient food can carry several of them.
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Figure 3

Plain Yogurt

Milk sugar, fermentation and calcium.

Nutrition Facts

Plain whole-milk yogurt · typical values, illustrative, not a specific brand

Serving size1 cup (245 g)

Calories150

% Daily Value

Total Fat8 g10%
Saturated Fat5 g25%
Trans Fat0 g
Cholesterol30 mg10%
Sodium115 mg5%
Total Carbohydrate11 g4%
Dietary Fiber0 g0%
Total Sugars11 g
Includes 0 g Added Sugars0%
Protein9 g
Vitamin D0 mcg0%
Calcium300 mg23%
Iron0 mg0%
Potassium380 mg8%

Ingredients: Cultured pasteurized whole milk. Contains live and active cultures.

Line by line
Label lineBranchWhat it is hereLesson
Total Sugars 11 g, Added 0 gCarbohydratesall of it is lactose, the sugar already in milkCarbohydrates, Fig. 4
CulturedProcessing · Flavoringsbacteria turn some lactose into lactic acid, which thickens the milk protein and tastes sourProcessing, Fig. 6 · Flavorings, Fig. 4
PasteurizedProcessingmilk heated to kill harmful microbes before the cultures are addedProcessing, Fig. 6
Saturated Fat, CholesterolFats · Lipidsmilk fat is mostly saturated; cholesterol is a separate sterolFats, Fig. 4 · Other Lipids, Fig. 3
Protein 9 gProteincasein and whey, a complete protein rich in leucineProtein, Fig. 7
Calcium 300 mgMajor mineralscalcium ions, about a quarter of the Daily ValueMajor Minerals, Fig. 5

Key molecules in yogurt

1

LactoseC₁₂H₂₂O₁₁ · milk sugar

2

Lactic acidC₃H₆O₃ · made by the cultures

3

LeucineC₆H₁₃NO₂ · in milk protein

4 · Calcium ionCa²⁺

  1. 1Lactose is a double sugar. The cultures use some of it up, and live cultures help digest the rest, so many people who are lactose intolerant manage yogurt better than milk.
  2. 2Lactic acid is what the cultures make from lactose. Its acid sets the milk proteins into a soft gel and gives yogurt its tang.
  3. 3Milk protein is rich in leucine, the amino acid that most strongly switches on muscle building.
  4. 4Calcium in yogurt is well absorbed, and one cup gives about a quarter of the Daily Value.

carbon hydrogen oxygen nitrogen calcium · every molecule on this page is drawn at the same scale with every hydrogen shown · tap one to enlarge it

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Figure 4

Canned Beans

Salt, an additive, fiber and the antinutrients the can deals with.

Nutrition Facts

Canned black beans, drained · typical values, illustrative, not a specific brand

Serving size½ cup (130 g)

Calories110

% Daily Value

Total Fat0.5 g1%
Saturated Fat0 g0%
Trans Fat0 g
Cholesterol0 mg0%
Sodium400 mg17%
Total Carbohydrate20 g7%
Dietary Fiber7 g25%
Total Sugars0 g
Includes 0 g Added Sugars0%
Protein7 g
Vitamin D0 mcg0%
Calcium40 mg3%
Iron1.8 mg10%
Potassium350 mg7%

Ingredients: Black beans, water, salt, calcium chloride.

Line by line
Label lineBranchWhat it is hereLesson
Sodium 400 mg, from saltMajor mineralsabout 1 g of salt; draining and rinsing washes some away, and “no salt added” cans have far lessMajor Minerals, Fig. 8
Calcium chlorideAdditivesa firming agent (E509) that keeps the beans from going mushyAdditives and E-Numbers, Fig. 2
Dietary Fiber 7 gCarbohydratessoluble and insoluble fiber, a quarter of the Daily ValueCarbohydrates, Fig. 6
Protein 7 gProteinrich in lysine, low in methionine, so beans pair well with grainsProtein, Fig. 7
Iron 1.8 mgTrace mineralsnonheme iron, better absorbed with vitamin C in the same mealTrace Minerals, Fig. 3
Not printed: phytate, lectinsAntinutrientsphytate binds some iron and zinc; the canning heat destroys the lectins that make raw beans unsafeAntinutrients, Fig. 3 · Antinutrients, Fig. 6

Key molecules in canned beans

1 · Sodium ionNa⁺

1 · Chloride ionCl⁻

2

LysineC₆H₁₄N₂O₂

3

Phytic acidC₆H₁₈O₂₄P₆

4 · Iron ionFe²⁺

  1. 1Salt dissolves into sodium and chloride ions. The sodium line counts only the sodium.
  2. 2Beans are rich in lysine, the amino acid grains are short of, which is why beans and rice or bread make a complete protein together.
  3. 3Phytic acid’s six phosphate groups grip iron, zinc and calcium ions. Soaking and cooking lower it somewhat.
  4. 4Plant iron is absorbed as Fe²⁺. Vitamin C turns more of it into this form, and phytate and tea tannins hold it back.

carbon hydrogen oxygen nitrogen phosphorus sodium chlorine iron · every molecule on this page is drawn at the same scale with every hydrogen shown · tap one to enlarge it

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Figure 5

Rolled Oats

One ingredient: starch, beta-glucan fiber and a processing step.

Nutrition Facts

Rolled oats, dry · typical values, illustrative, not a specific brand

Serving size½ cup (40 g)

Calories150

% Daily Value

Total Fat3 g4%
Saturated Fat0.5 g3%
Trans Fat0 g
Cholesterol0 mg0%
Sodium0 mg0%
Total Carbohydrate27 g10%
Dietary Fiber4 g14%
Total Sugars1 g
Includes 0 g Added Sugars0%
Protein5 g
Vitamin D0 mcg0%
Calcium20 mg2%
Iron1.7 mg10%
Potassium150 mg3%

Ingredients: Whole grain rolled oats.

Line by line
Label lineBranchWhat it is hereLesson
Total Carbohydrate 27 gCarbohydratesmostly starch: long chains of alpha-glucose that your enzymes break downCarbohydrates, Fig. 5
Dietary Fiber 4 gCarbohydratesincludes beta-glucan, a soluble fiber made of beta-glucoseCarbohydrates, Fig. 6
RolledProcessingoats are steamed to stop a fat-splitting enzyme that would turn them rancid, then flattened between rollersProcessing, Fig. 2
Iron 1.7 mgTrace mineralsnonheme plant ironTrace Minerals, Fig. 3
Protein 5 gProteingrain protein: low in lysine, so oats pair well with milk, yogurt or beansProtein, Fig. 7
Not printed: phytateAntinutrientswhole grains keep the phytate-rich outer layers; soaking overnight lowers it a littleAntinutrients, Fig. 8

Starch and fiber: the same sugar, a different link

1

Alpha-glucosebuilds starch

2

Beta-glucosebuilds beta-glucan

3

Beta linktwo beta-glucose units

  1. 1Starch is chains of alpha-glucose. Your amylase enzymes cut those links, so starch becomes blood glucose.
  2. 2Beta-glucose differs only in which way one OH group points.
  3. 3Beta links like this one resist your enzymes, so oat beta-glucan reaches the gut as soluble fiber. It thickens into a gel, and about 3 g a day from oats, in a diet low in saturated fat, can lower LDL cholesterol; the US FDA allows a heart-health claim on that basis.

carbon hydrogen oxygen · every molecule on this page is drawn at the same scale with every hydrogen shown · tap one to enlarge it

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Figure 6

Olive Oil

Almost pure fat, plus vitamin E and polyphenols.

Nutrition Facts

Extra virgin olive oil · typical values, illustrative, not a specific brand

Serving size1 tbsp (14 g)

Calories120

% Daily Value

Total Fat14 g18%
Saturated Fat2 g10%
Trans Fat0 g
Polyunsaturated Fat1.5 g
Monounsaturated Fat10 g
Cholesterol0 mg0%
Sodium0 mg0%
Total Carbohydrate0 g0%
Protein0 g
Vitamin E (optional line)2 mg13%

Ingredients: Extra virgin olive oil.

Line by line
Label lineBranchWhat it is hereLesson
Calories 120Fats14 g of fat × 9 kcal per gram is 126; labels round to the nearest 10Fats, Fig. 1
Monounsaturated Fat 10 gFatsmostly oleic acid, the omega-9 fatty acid with one cis double bondFats, Fig. 5 · Fats, Fig. 6
Cholesterol 0 mgLipidsplants make no cholesterol; olive oil has plant sterols insteadOther Lipids, Fig. 3
Vitamin EFat-soluble vitaminsalpha-tocopherol, an antioxidant that protects the oil and your cell membranesFat-Soluble Vitamins, Fig. 5
Extra virginProcessingpressed from olives with no heat-and-solvent refining, so it keeps more polyphenolsProcessing, Fig. 7
Not printed: polyphenolsBioactives · Flavoringshydroxytyrosol and related polyphenols, also behind the peppery, bitter taste of fresh oilBioactives, Fig. 3 · Flavorings, Fig. 3

Key molecules in olive oil

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3

Alpha-tocopherolC₂₉H₅₀O₂ · vitamin E

4

HydroxytyrosolC₈H₁₀O₃ · polyphenol

  1. 1Every fat and oil is triglycerides: glycerol, shaded, holding three fatty acids (Fats, Figure 3).
  2. 2One cis double bond, drawn in orange, kinks the chain. In olive oil most chains are oleic acid, which is why it stays liquid.
  3. 3Vitamin E dissolves in the oil. Its OH group gives up a hydrogen to stop fats from going rancid.
  4. 4Hydroxytyrosol’s two neighbouring OH groups make it an antioxidant. The EU allows a claim that olive oil polyphenols help protect blood lipids from oxidation, at about 5 mg a day in 20 g of oil with enough of them.

carbon hydrogen oxygen · every molecule on this page is drawn at the same scale with every hydrogen shown · tap one to enlarge it

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Figure 7

Simple Bread

Enriched flour, yeast, salt and the crust.

Nutrition Facts

Simple white sandwich bread · typical values, illustrative, not a specific brand

Serving size1 slice (30 g)

Calories80

% Daily Value

Total Fat1 g1%
Saturated Fat0 g0%
Trans Fat0 g
Cholesterol0 mg0%
Sodium150 mg7%
Total Carbohydrate15 g5%
Dietary Fiber1 g4%
Total Sugars1 g
Includes 0 g Added Sugars0%
Protein3 g
Vitamin D0 mcg0%
Calcium20 mg2%
Iron1 mg6%
Potassium30 mg0%

Ingredients: Enriched wheat flour (wheat flour, niacin, reduced iron, thiamin mononitrate, riboflavin, folic acid), water, yeast, salt.

Contains: wheat.

Line by line
Label lineBranchWhat it is hereLesson
Enriched wheat flourMicronutrientsmilling removes the bran and germ, so B vitamins and iron are added backWater-Soluble Vitamins, Fig. 8
Folic acidWater-soluble vitaminsrequired in US enriched grain since 1998 to help prevent neural tube defectsWater-Soluble Vitamins, Fig. 5
Reduced ironTrace mineralsfine iron powder added to the flourTrace Minerals, Fig. 8
YeastWater and Ethanol · Processingyeast ferments sugar into carbon dioxide, which raises the dough, and ethanol, which bakes offWater and Ethanol, Fig. 5
CrustProcessingbrowning makes flavor and a little acrylamide; toast to golden, not dark brownProcessing, Fig. 3
Salt, Sodium 150 mgMajor mineralsbread is one of the biggest sources of sodium in many diets because people eat it oftenMajor Minerals, Fig. 8
Contains: wheatProteinwheat is a major allergen; its gluten proteins form the stretchy network that traps the gasProtein, Fig. 5

Key molecules in bread

1
2

EthanolC₂H₆O

2

Carbon dioxideCO₂

3

AcrylamideC₃H₅NO · in the crust

  1. 1Folic acid is the stable, added form of folate. Your body converts it to the active form.
  2. 2Yeast turns each glucose into two ethanol and two carbon dioxide. The gas makes the holes; the ethanol evaporates in the oven.
  3. 3Acrylamide forms when the amino acid asparagine meets sugar in dry heat. Most of it is in the crust and in dark toast.

carbon hydrogen oxygen nitrogen · every molecule on this page is drawn at the same scale with every hydrogen shown · tap one to enlarge it

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Figure 8

Putting It All Together

The five foods in one table, plus a few things worth knowing.

Five foods at a glance
FoodLines that tell the storyBranches it touchesNot on the label
Plain yogurt11 g sugars, 0 g added; calcium 23%Carbohydrates, Fats, Lipids, Protein, Major minerals, Processing, Flavoringslive cultures
Canned beanssodium 17%, fiber 25%, calcium chlorideCarbohydrates, Protein, Major and Trace minerals, Additivesphytate; lectins destroyed by canning
Rolled oatsfiber 4 g, no sodium, one ingredientCarbohydrates, Protein, Trace minerals, Processingbeta-glucan amount, phytate
Olive oil14 g fat, 10 g of it monounsaturatedFats, Lipids, Fat-soluble vitamins, Processingpolyphenols such as hydroxytyrosol
White breadenriched flour, folic acid, saltCarbohydrates, Protein, Water-soluble vitamins, Trace and Major minerals, Water and Ethanol, Processingacrylamide in the crust
Worth knowing

Labels round

A nutrient under 0.5 g per serving can be listed as 0 g, so “0 g trans fat” can still mean a little. Calories above 50 are rounded to the nearest 10, and sodium above 140 mg to the nearest 10 mg.

Serving sizes are reference amounts

The serving is based on how much people typically eat at one time, not on how much you should eat. Compare foods per 100 g when servings differ.

Whole foods often have no label

Fresh fruit, vegetables and raw single-ingredient meat and fish do not need a Nutrition Facts panel. Databases such as USDA FoodData Central list typical values.

Every number here is typical

Brands and batches differ. The labels in this lesson are illustrative averages, so always read the one on the package in your hand.

General education, not medical advice. Typical values are rounded averages in the style of USDA FoodData Central and US FDA label rules (21 CFR 101.9), not any brand’s label.

Quiz

Tap a question to see the answer

1. How many calories does a gram of protein, carbohydrate, fat and alcohol give?

About 4, 4, 9 and 7 (Figure 2; Fats, Water and Ethanol).

2. A tablespoon of olive oil has 14 g of fat. Why does the label say about 120 calories?

14 g × 9 kcal per gram is 126, and labels round calories above 50 to the nearest 10 (Figure 6).

3. Plain yogurt lists 11 g of total sugars but 0 g added sugars. Where does the sugar come from?

Lactose, the sugar naturally in milk (Figure 3; Carbohydrates, Figure 8).

4. What makes yogurt thick and tangy?

Cultures turn lactose into lactic acid, which sets the milk proteins into a gel and tastes sour (Figure 3; Processing, Figure 6).

5. Why is cholesterol a separate line in milligrams rather than part of Total Fat?

Cholesterol is a sterol built from four rings, not a triglyceride, and it has no calories (Lipids, Figure 3).

6. A can of beans lists 400 mg of sodium per serving. Roughly how much salt is that?

About 1 g, since salt is about 2.5 times the sodium figure (Figure 4; Major Minerals, Figure 8).

7. What is calcium chloride doing in canned beans?

It is a firming agent, an additive (E509) that keeps the beans from going soft (Figure 4; Additives).

8. Why can you eat canned kidney beans straight from the can but not raw or undercooked ones?

The canning heat destroys the lectins that cause illness (Figure 4; Antinutrients, Figure 6).

9. Why do beans and grains make a good protein pair?

Beans are rich in lysine but low in methionine, and grains are the reverse (Figure 4; Protein, Figure 7).

10. Starch and oat beta-glucan are both made of glucose. Why is one fiber?

Beta-glucan uses beta links that human enzymes cannot cut, while starch uses alpha links that amylase breaks (Figure 5; Carbohydrates, Figure 6).

11. Why are oats steamed before they are rolled?

To stop a fat-splitting enzyme that would make them go rancid (Figure 5; Processing).

12. What does “enriched” mean on flour, and which added vitamin helps prevent neural tube defects?

B vitamins and iron are added back after milling; folic acid prevents neural tube defects (Figure 7; Water-Soluble Vitamins).

13. Where do the holes in bread come from?

Yeast ferments sugar into carbon dioxide gas, which the gluten network traps; the ethanol bakes off (Figure 7; Water and Ethanol, Figure 5).

14. Why toast bread to golden rather than dark brown?

Browning forms acrylamide, and darker toast has more of it (Figure 7; Processing, Figure 3).

15. Which fat-soluble vitamin does olive oil supply, and what does it protect?

Vitamin E, alpha-tocopherol, which protects fats in the oil and in your cell membranes from oxidation (Figure 6; Fat-Soluble Vitamins, Figure 5).

16. Which two Non-Nutrients lessons cover compounds that never get their own label line?

Antinutrients and bioactives, such as phytate and polyphenols (Figures 1 and 2).

17. Does “0 g trans fat” guarantee there is none?

No. Less than 0.5 g per serving can be listed as 0 g (Figure 8).

18. On the % Daily Value column, what counts as low and what counts as high?

5% or less is low and 20% or more is high (Figure 2).

Next lesson: Module 5 · Food Label Tree →