LeClaire Institute

Module 2 · Trace Minerals

The last micronutrient lesson covers the nine trace minerals, needed in amounts under 20 mg a day: iron, copper, zinc, selenium, iodine, fluoride, manganese, chromium and molybdenum. Each is shown as an ion or in a molecule that carries it, such as heme, selenocysteine and thyroxine, in ball-and-stick form, with what it does, what happens with too little or too much, and where to find it in whole foods. The figure is on the left and the lesson script on the right; the quiz is at the end.

Previous lesson: Module 2 · Major Minerals

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Figure 1

The Trace Mineral Family Tree

Nine minerals needed in amounts under 20 mg a day.

Small amounts, big jobs
Under 20 mg a dayTrace minerals are needed in milligrams or micrograms, but each is essential: most sit at the active center of enzymes or other proteins.
A narrow windowThe gap between enough and too much is small for several of them, notably iron, selenium and fluoride, so food is safer than high-dose supplements.
Soil and food choice matterThe selenium and iodine in plant foods depend on the soil where they grew; iron and zinc absorption depends on what else is in the meal (Figure 8).
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Figure 2

Iron and Heme

Two charges, and iron held in a ring.

Iron

Two forms of iron ion

Ferrous ironFe²⁺

Ferric ironFe³⁺

Sodium, for scaleNa⁺

Iron switches easily between Fe²⁺ and Fe³⁺ by gaining or losing one electron. That is what makes it useful for carrying oxygen and electrons, and also why loose iron can be harmful: it can spark damaging reactions, so the body keeps it bound to proteins.

Heme, C₃₄H₃₂FeN₄O₄

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  1. 1An iron atom held at the center…
  2. 2…by four nitrogens of a porphyrin ring, four linked five-membered rings, the same plan as the corrin ring of vitamin B12 and the ring around magnesium in chlorophyll.
  3. 3Two acid side chains. In hemoglobin, oxygen binds to the iron from above.

Heme iron comes only from animal foods (meat, poultry, fish), inside hemoglobin and myoglobin; it is absorbed well. Nonheme iron, mostly Fe³⁺, is the form in plants, eggs, dairy and fortified foods; it is absorbed less well and depends on the rest of the meal.

iron carbon hydrogen oxygen nitrogen · every molecule and ion on this page is drawn at the same scale with every hydrogen shown · tap one to enlarge it

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Figure 3

Iron in Your Body

Absorption, use, storage and recycling.

How your body handles iron
  1. Absorbed in the upper small intestineheme iron is taken up whole; nonheme Fe³⁺ must first be changed to Fe²⁺, which vitamin C helps with
  2. Carried by transferrina blood protein that holds iron safely
  3. Usedabout 70% of body iron is in hemoglobin, carrying oxygen in red blood cells, and myoglobin in muscle; a little is in enzymes
  4. Stored as ferritinin the liver, spleen and bone marrow
  5. Recycled, not excretedold red cells are broken down and their iron reused; the body has no active way to get rid of extra iron, so it controls how much it absorbs

Iron

In your body: RDA 8 mg for men and for women over 50; 18 mg for women 19–50, because of menstrual losses; 27 mg in pregnancy. Vegetarians may need about 1.8 times as much, because nonheme iron is less well absorbed.

Too little: The most common nutrient deficiency in the world. Stores run down first, then iron-deficiency anemia: tiredness, pale skin, shortness of breath, poor concentration.

Too much: Upper limit 45 mg a day for adults; supplements above it often upset the stomach. Accidental overdose of iron supplements is dangerous for young children, so keep them out of reach. Some people inherit a tendency to absorb too much (hemochromatosis).

Found in: Heme: beef, lamb, oysters, clams, sardines, dark-meat poultry. Nonheme: lentils, beans, tofu, spinach, pumpkin seeds, dried apricots.

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Figure 4

Zinc and Copper

Enzyme metals that compete with each other.

Zinc

Zinc ion, Zn²⁺

Zinc gives up two electrons and, unlike iron and copper, never changes charge. It is used to hold proteins in shape (“zinc fingers” that grip DNA) and as the working part of many enzymes.

In your body: Needed by about 100 enzymes; for immune function, wound healing, growth, DNA and protein making, and taste and smell. RDA 11 mg (men), 8 mg (women).

Too little: Slow growth in children, loss of appetite, poor wound healing, more infections, hair loss, loss of taste.

Too much: Upper limit 40 mg a day. Long-term high-dose supplements cause copper deficiency.

Found in: Oysters (by far the richest), beef, crab, lobster, pork, chicken, pumpkin seeds, beans, chickpeas, cashews, yogurt. Phytate in whole grains and beans lowers absorption (Figure 8).

Copper

Copper ion, Cu²⁺

Like iron, copper switches between two charges (Cu⁺ and Cu²⁺), so it works in enzymes that move electrons.

In your body: Helps make energy, connective tissue and the pigment melanin; needed to move iron out of storage, so a shortage can cause anemia. RDA 900 µg.

Too little: Rare: anemia, low white cells, nerve problems; often linked to high-dose zinc supplements or stomach surgery.

Too much: Upper limit 10 mg a day; very high doses damage the liver.

Found in: Oysters, liver, crab, cashews, sunflower seeds, lentils, mushrooms, dark chocolate, whole grains.

zinc copper · ions drawn to the same scale as Figure 2 · tap one to enlarge it

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Figure 5

Selenium

The 21st amino acid.

Selenium

Selenium in amino acids

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Cysteinewith sulfur

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Selenocysteinewith selenium

Selenomethionine, the main form in plant foods

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  1. 1Cysteine has sulfur (yellow)…
  2. 2…selenocysteine has selenium (orange), the element just below sulfur in the periodic table. It is called the 21st amino acid and is built into about 25 human selenoproteins.
  3. 3Plants take up selenium from soil and build it into selenomethionine in place of methionine.

In your body: Selenoproteins include antioxidant enzymes (glutathione peroxidases) and enzymes that activate thyroid hormone. RDA 55 µg.

Too little: Rare in the US, where soils and a varied food supply provide enough; in low-selenium regions it has caused a heart disease (Keshan disease).

Too much: Upper limit 400 µg a day. Too much (selenosis) causes garlic-smelling breath, brittle hair and nails, and skin rashes. One Brazil nut can contain more than a day’s needs, so eat them only occasionally.

Found in: Brazil nuts, tuna, sardines, shrimp, eggs, chicken, turkey, beef, whole grains; plant content depends on the soil.

carbon hydrogen oxygen nitrogen sulfur selenium · every molecule on this page is drawn at the same scale with every hydrogen shown · tap one to enlarge it

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Figure 6

Iodine and the Thyroid

Iodide, T4 and T3.

Iodine

Iodide, I⁻

Iodine is taken in as iodide, a large negative ion. The thyroid gland traps it from the blood and builds it into thyroid hormones.

The thyroid hormones

Thyroxine (T4), C₁₅H₁₁I₄NO₄

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Triiodothyronine (T3), C₁₅H₁₂I₃NO₄

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  1. 1Two iodines (purple) on the inner ring…
  2. 2…and two on the outer ring: four in all, so thyroxine is called T4.
  3. 3It is built from the amino acid tyrosine.
  4. 4In body tissues one outer iodine is removed (by a selenium enzyme), giving T3, the active hormone.

In your body: Thyroid hormones set the body’s metabolic rate and are essential for brain and bone development before birth and in infancy. RDA 150 µg; 220 µg in pregnancy.

Too little: Goiter (an enlarged thyroid) and hypothyroidism. In pregnancy, deficiency can impair the baby’s brain development; worldwide it is a leading preventable cause of intellectual disability.

Too much: Upper limit 1,100 µg a day. Too much can also disturb the thyroid, causing goiter or an over- or underactive thyroid.

Found in: Seaweed (amounts vary widely), cod, shrimp, tuna, milk, yogurt, cheese, eggs, and iodized salt. Sea salt, kosher salt and most salt in processed foods are not usually iodized.

iodine carbon hydrogen oxygen nitrogen · every molecule on this page is drawn at the same scale with every hydrogen shown · tap one to enlarge it

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Figure 7

Fluoride, Manganese, Chromium, Molybdenum

Four more, in brief.

Fluoride, manganese, chromium, molybdenum

Four more trace minerals

FluorideF⁻

ManganeseMn²⁺

ChromiumCr³⁺

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MolybdateMoO₄²⁻

  1. 1Molybdenum (teal) arrives mostly as molybdate; the body builds it into a cofactor for four enzymes.
At a glance
MineralIn your bodyIntake (adults)Too little / too muchWhole-food sources
Fluoridereplaces OH in tooth enamel to form fluorapatite, which resists acidAI 4 mg (men), 3 mg (women); upper limit 10 mgmore tooth decay / white flecks on teeth (fluorosis) if too much while teeth formfluoridated water, tea, seafood
Manganeseenzymes for bone, carbohydrate and amino-acid use, and an antioxidant enzyme (MnSOD)AI 2.3 mg (men), 1.8 mg (women); upper limit 11 mgrare / nerve damage, mostly from breathing it in at work or from well waterwhole grains, nuts, beans, tea, leafy greens, pineapple
Chromiummay help insulin work; supplements show little clear benefitAI 35 µg (men), 25 µg (women)rare / no harm known from foodbroccoli, potatoes, whole grains, beef, green beans
Molybdenumcofactor for four enzymes, including sulfite oxidaseRDA 45 µg; upper limit 2,000 µgessentially unknown / rarebeans, lentils, peas, whole grains, nuts

fluorine manganese chromium molybdenum oxygen · every molecule on this page is drawn at the same scale with every hydrogen shown · tap one to enlarge it

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Figure 8

Absorption and Labels

What helps and blocks absorption, and Daily Values.

Helpers and blockers
In the same mealEffect
Vitamin C (peppers, citrus, tomatoes, broccoli)increases nonheme iron absorption
Meat, poultry or fishincreases nonheme iron absorption from the rest of the meal
Phytate (whole grains, beans, nuts, seeds)lowers iron and zinc absorption (Antinutrients)
Polyphenols (tea, coffee)lower nonheme iron absorption; drink them between meals if iron is low
Calcium (dairy, supplements)lowers iron absorption a little
High-dose zinc supplementslower copper absorption
Making minerals easier to absorb
Soaking, sprouting, fermentingSoaking beans, sprouting grains and leavening bread with yeast or sourdough break down phytate and free more iron and zinc.
Cast-iron pansCooking acidic foods such as tomato sauce in cast iron adds some iron to the food.
Daily Values
On the labelDaily Value
Iron (required)18 mg
Zinc11 mg
Copper0.9 mg
Selenium55 µg
Iodine150 µg
Manganese2.3 mg
Chromium35 µg
Molybdenum45 µg
On the label
Iron is requiredIron must appear on every Nutrition Facts label; the other trace minerals appear only if added or a claim is made. Fluoride has no Daily Value.
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Figure 9

Where trace minerals are used in the body

Tap a number on the cell to see what that part does; the list below it gives every other place in the body.

Tiny amounts, big jobs: oxygen transport, enzymes, thyroid hormones and teeth.

Inside a cell

A typical animal cell, not to scale. Real cells hold thousands of ribosomes and hundreds of mitochondria.

Tiny amounts of iron, zinc, copper, selenium, iodine and manganese power key enzymes.

  1. Iron in heme and iron-sulfur clusters, and copper, carry electrons in the chain that makes ATP. Heme itself is partly made here.
  2. Zinc fingers are protein parts that hold zinc and grip DNA to control genes.
  3. Selenium in glutathione peroxidase and zinc and copper in superoxide dismutase clear harmful oxidants; ferritin stores iron.
  4. Manganese superoxide dismutase protects the mitochondria from the oxidants they make.
  5. Carriers such as DMT1 for iron and ZIP proteins for zinc bring trace minerals in.
  6. Thyroid cells pump iodide in, then an enzyme at the cell's outer surface attaches it to thyroglobulin, a protein made in the rough ER, to make thyroid hormone.

Everywhere else in the body

Each item, the places outside the cell where it is found or used, and what it does there.

Iron

  • Red blood cells. About two thirds of body iron, in hemoglobin, which carries oxygen.
  • Muscle. Myoglobin, another iron protein, holds oxygen.
  • Bone marrow. Builds iron into new red blood cells.
  • Spleen. Recycles iron from old red cells.
  • Liver. Stores iron as ferritin and makes hepcidin, the hormone that sets how much is absorbed.
  • Small intestine (duodenum). Absorbs iron; heme iron from meat best.
  • Blood. Transferrin carries iron between tissues.
  • Brain. Needed for myelin and to make dopamine.
  • Placenta. Passes iron to the baby; needs rise in pregnancy.
  • Uterus. Periods lose iron each month.

Zinc

  • Muscle and bone. Hold most of the body’s zinc.
  • Skin. Needed for wound healing.
  • Immune system. Needed for immune cells, including in the thymus.
  • Eyes. High levels in the retina.
  • Prostate and semen. Some of the highest levels.
  • Pancreas. Insulin is stored packed with zinc.
  • Tongue and nose. A shortage dulls taste and smell.
  • Small intestine. Absorbs zinc; phytate in grains and beans reduces it.

Copper

  • Liver. Stores copper, makes ceruloplasmin and sends extra out in bile.
  • Blood. Ceruloplasmin helps load iron onto transferrin.
  • Brain. Needed to make noradrenaline and for myelin.
  • Skin and hair. Needed to make melanin, the pigment.
  • Blood vessels, skin and bone. A copper enzyme links collagen and elastin fibers.

Selenium

  • Thyroid. Holds the most per gram; selenium enzymes switch thyroid hormone on and off.
  • Muscle. Holds the largest share of the total.
  • Testes and sperm. Needed for healthy sperm.
  • Blood. Selenoprotein P carries it to tissues.
  • Immune system. Needed for immune cells.

Iodine

  • Thyroid. Holds most of the body’s iodine and builds it into the hormones T4 and T3, which set how fast you use energy.
  • Fetal brain. Needs the mother’s thyroid hormone, so iodine matters in pregnancy.
  • Breasts, salivary glands and stomach. Also take iodide up; breast milk passes it to the baby.
  • Kidneys. Send most iodine out in urine.

Fluoride

  • Teeth. Turns enamel into harder, acid-resistant fluorapatite; saliva fluoride helps repair it.
  • Bones. Hold nearly all body fluoride; too much over years causes skeletal fluorosis.

Manganese, chromium and molybdenum

  • Bone. Manganese helps build bone and cartilage.
  • Liver, kidneys and pancreas. Hold the most manganese.
  • Brain. Too much manganese, usually breathed in at work, damages it.
  • Blood. Chromium may help insulin work; the evidence is weak.
  • Liver and kidneys. Molybdenum enzymes break down sulfites and make uric acid.
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Figure 10

Putting It All Together

The whole lesson in one table, plus a few extras.

The nine trace minerals
MineralMain jobToo littleWhole-food sources
Ironcarries oxygen (hemoglobin)anemia, fatiguered meat, shellfish, lentils, beans, spinach
Copperiron use, energy, connective tissueanemia (rare)oysters, liver, nuts, seeds, cocoa
Zincenzymes, immunity, healingslow growth and healing, infectionsoysters, beef, crab, pumpkin seeds, beans
Seleniumantioxidant enzymes, thyroidrare in USBrazil nuts, fish, eggs, poultry
Iodinethyroid hormonesgoiter; brain development in pregnancyseaweed, fish, dairy, eggs, iodized salt
Fluoridestrong tooth enameltooth decayfluoridated water, tea
Manganeseenzymes, bonerarewhole grains, nuts, tea
Chromiummay help insulinrarebroccoli, whole grains
Molybdenumfour enzymesunknownbeans, lentils, grains
Worth knowing

The same ring, three times

Heme, the corrin ring of vitamin B12 and chlorophyll all hold a metal (iron, cobalt, magnesium) at the center of a ring of four nitrogen-containing rings.

Blood donors and iron

Each blood donation removes about 200–250 mg of iron, which is why donors are screened for anemia.

Iodized salt

Adding iodine to salt, started in the US in 1924, all but eliminated goiter in the country.

General education, not medical advice. Facts follow mainstream sources (US Dietary Guidelines, NIH Office of Dietary Supplements, standard biochemistry and food-science texts).

Quiz

Tap a question to see the answer

1. What makes a mineral a trace mineral?

You need it in amounts under about 20 mg a day (Figure 1).

2. What are the two charges iron switches between?

Fe²⁺ (ferrous) and Fe³⁺ (ferric) (Figure 2).

3. What holds the iron in heme?

Four nitrogens of a porphyrin ring (Figure 2).

4. Which foods supply heme iron?

Animal foods: meat, poultry and fish (Figure 2).

5. How does the body get rid of extra iron?

It has no active way; it controls how much it absorbs and recycles iron from old red cells (Figure 3).

6. Why do women 19–50 need more iron than men?

To replace menstrual losses (Figure 3).

7. What can long-term high-dose zinc supplements cause?

Copper deficiency (Figure 4).

8. Which food is by far the richest in zinc?

Oysters (Figure 4).

9. What is selenocysteine?

Cysteine with selenium in place of sulfur, the 21st amino acid (Figure 5).

10. Why eat Brazil nuts only occasionally?

One nut can exceed a day’s selenium needs; too much causes selenosis (Figure 5).

11. How many iodine atoms are in thyroxine (T4)? In T3?

Four; three (Figure 6).

12. Name one thing that helps and one that hinders nonheme iron absorption.

Helps: vitamin C or meat in the meal. Hinders: phytate, tea or coffee polyphenols, calcium (Figure 8).

Next lesson: Module 3 · Additives and E-Numbers →