LeClaire Institute

Module 3 · Additives and E-Numbers

Additives are substances added to food to do a job: color, preserve, thicken, emulsify, sweeten or season. This lesson groups them by job and shows the key molecules, from citric acid to sucralose, with how they are labeled and regulated in the US and EU. Ultra-processed foods appear here because that is where most additives are used.

Previous lesson: Module 2 · Trace Minerals

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Figure 1

The Additive Family Tree

Additives grouped by the job they do.

Reading the numbers
E for EuropeAn E-number is the code an additive gets once it has passed an EU safety review. The hundreds digit roughly tells you the job: 1 colors, 2 preservatives, 3 antioxidants and acids, 4 thickeners and emulsifiers, 5 acidity regulators and anti-caking agents, 6 flavor enhancers, 9 sweeteners and glazes.
Grouped by jobSome additives break the pattern: lecithin (E322) is an emulsifier, and acids turn up in both the 300s and 500s. That is why this lesson groups them by what they do.
Not all syntheticMany additives are ordinary food compounds: vitamin C (E300), citric acid (E330), pectin (E440), lecithin from egg yolk or soy (E322).
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Figure 2

What Counts as an Additive

Definition, labels and approval, with citric acid as the first example.

What counts as an additive
Added on purposeAn additive is a substance added to food to do a technical job: color it, keep it from spoiling, thicken it, sweeten it, or stop it from clumping. Ordinary ingredients such as flour, salt and sugar are not usually called additives.
Listed by weightThe ingredient list runs from most to least by weight, so additives, used in small amounts, are usually near the end.
Same molecule, either sourceAn additive is the same molecule whether it was squeezed from a fruit or made in a factory; what matters is the amount and the food it is in.

Citric acid, E330, C₆H₈O₇

1112
  1. 1Three acid (COOH) groups give the sour taste…
  2. 2…around a central carbon with an OH.

What it is: The acid that makes lemons and limes sour. Added to drinks, jams and canned foods for tartness, to adjust acidity, and to help stop color and flavor changes.

In your body: Your cells make and use it every day: it is the first compound in the citric acid cycle, the main route for releasing energy from food.

Too much: Safe at the levels in food. Like any acid, frequent sour drinks can wear tooth enamel.

Found in: Lemons, limes, oranges, grapefruit, berries, tomatoes. Most added citric acid is made by fermenting sugar with a mold (Aspergillus niger).

Rules and labels
TopicUnited StatesEuropean Union
ApprovalFDA review as a food or color additive, or “generally recognized as safe” (GRAS)EFSA safety review, then an E-number
On the labelby name, such as “sodium benzoate” or “Red 40”by job plus name or E-number, such as “preservative: E211”
Numbersnot used on labelsE-numbers; the worldwide Codex INS number is the same digits without the E (citric acid is INS 330)

carbon hydrogen oxygen · every molecule on this page is drawn at the same scale with every hydrogen shown · tap one to enlarge it

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Figure 3

Colors

From turmeric to azo dyes and titanium dioxide.

Colors from plants

Curcumin, E100, C₂₁H₂₀O₆

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  1. 1Two rings at the ends…
  2. 2…joined by a long chain of alternating single and double bonds, which absorbs blue light, so it looks yellow.

What it is: The yellow pigment of turmeric, used to color mustard, cheese, butter and drinks.

In your body: Poorly absorbed from food; it gives color, not a nutrient.

Found in: Turmeric root. Other colors from whole foods include beet red (betanin, E162), paprika (E160c) and beta-carotene (E160a).

Synthetic colors

Allura Red AC, Red 40, E129

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  1. 1An N=N “azo” bond joins two ring systems; azo dyes are the largest group of synthetic food colors.
  2. 2Charged sulfonate groups make it dissolve in water.

What it is: A synthetic red dye made from petroleum, used in candy, drinks, cereals and snacks; sold as the sodium salt.

Too much: In the EU, foods with Red 40 or five other synthetic colors must carry the warning “may have an adverse effect on activity and attention in children.” In 2025 the FDA said it plans to phase out petroleum-based dyes, and it revoked the use of Red 3 in food from 2027.

Found in: Not in whole foods; ultra-processed foods only.

Titanium dioxide, E171, TiO₂

What it is: A bright white mineral pigment, used to whiten candy coatings, frostings and chewing gum. In food it is a fine powder of tiny crystals; one unit is shown.

Too much: Banned as a food additive in the EU from 2022, after EFSA concluded it could no longer be considered safe because harm to DNA could not be ruled out. The US still permits it, up to 1% of a food’s weight.

Found in: Not in whole foods.

carbon hydrogen oxygen nitrogen sulfur titanium · every molecule on this page is drawn at the same scale with every hydrogen shown · tap one to enlarge it

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Figure 4

Preservatives

Stopping molds, yeasts and bacteria.

Against molds and yeasts

Sorbic acid and benzoic acid

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Sorbic acidE200; potassium sorbate E202

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Benzoic acidE210; sodium benzoate E211

  1. 1Both are weak acids. In acidic foods they stay uncharged, slip into mold and yeast cells and stop them growing.

What it is: The most common preservatives against molds and yeasts. Sorbates are used in cheese, baked goods and wine; benzoates in soft drinks, pickles and sauces.

In your body: Sorbic acid is broken down like a fatty acid; benzoic acid is joined to glycine in the liver and passed in urine.

Too much: Low toxicity at permitted levels. With vitamin C, heat and light, benzoate can form traces of benzene in drinks, so manufacturers reformulated many drinks after FDA testing in 2006–2007.

Found in: Sorbic acid was first found in rowan berries; benzoic acid occurs naturally in cranberries, cinnamon and cloves.

Curing meat

Nitrite, NO₂⁻

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  1. 1A nitrogen with two oxygens. Sodium nitrite is E250; nitrates (E251, E252) turn into nitrite.

What it is: The curing agent in bacon, ham, hot dogs and salami. It stops Clostridium botulinum, the bacterium that causes botulism, and gives cured meat its pink color and flavor.

Too much: Nitrite can react with amines in meat to form nitrosamines, especially at high heat such as frying bacon (see Processing). The WHO’s cancer agency classes processed meat as a cause of colorectal cancer.

Found in: Most nitrate in the diet comes from vegetables such as spinach, beets, celery and lettuce, where it comes packaged with vitamin C and other protective compounds. Meats labeled “uncured” are usually cured with celery powder, a natural source of nitrate.

Sulfites

Sulfur dioxide, SO₂, E220

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  1. 1Sulfur dioxide and sulfite salts (E220–E228) release the same active form.

What it is: Preservatives and antibrowning agents in wine, dried fruit such as apricots, and some potato products.

Too much: Can trigger asthma attacks in sensitive people. The US requires sulfites to be declared at 10 parts per million or more and bans them on fresh fruit and vegetables meant to be eaten raw. Sulfites also destroy thiamin (vitamin B1).

Found in: Formed in small amounts naturally during wine fermentation.

carbon hydrogen oxygen nitrogen sulfur · every molecule on this page is drawn at the same scale with every hydrogen shown · tap one to enlarge it

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Figure 5

Antioxidants and Acids

Stopping browning and rancidity, and adding tang.

Antioxidants from plants

Ascorbic acid, vitamin C, E300

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  1. 1The two OH groups on the double bond give up electrons, so vitamin C is oxidized first and the food is protected.

What it is: Added to stop cut fruit, juices and wine from browning, to help bread dough, and to cured meats, where it speeds curing and reduces nitrosamine formation.

Found in: Citrus, peppers, kiwi, strawberries, broccoli. Tocopherols (vitamin E, E306–E309) and rosemary extract (E392) are other antioxidants from plants.

Synthetic antioxidants

BHA and BHT

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BHAE320

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BHTE321

  1. 1An OH on a ring gives up a hydrogen to stop the chain reaction that turns fats rancid…
  2. 2…and the bulky tert-butyl groups keep the leftover molecule stable.

What it is: Synthetic antioxidants that keep fats and oils from going rancid, used in cereals, chewing gum, snack foods and fats.

Too much: Permitted in small amounts. The US National Toxicology Program lists BHA as “reasonably anticipated to be a human carcinogen,” based on high doses in animals; evidence in people is lacking.

Found in: Not in whole foods.

Acids

Phosphoric acid, E338

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  1. 1A phosphorus with four oxygens, three of them carrying a hydrogen.

What it is: Gives colas their sharp tang. Related phosphates (E339–E343, E450–E452) are used in processed meats, cheese and baking powder.

Too much: Phosphate additives are absorbed more completely than the phosphorus in whole foods, which matters for people with kidney disease (see Major Minerals). Other acids from foods: acetic acid (vinegar, E260), lactic acid (yogurt, E270), malic acid (apples, E296).

Found in: Phosphate is in every cell: meat, fish, dairy, beans, nuts and whole grains.

carbon hydrogen oxygen phosphorus · every molecule on this page is drawn at the same scale with every hydrogen shown · tap one to enlarge it

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Figure 6

Thickeners and Emulsifiers

Gels, gums and molecules with two ends.

Thickeners

Galacturonic acid, the building block of pectin

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  1. 1A sugar ring with an acid group. Pectin is long chains of these, which trap water and, with sugar and acid, set jam.

What it is: Pectin (E440) is a soluble fiber from plant cell walls, used to set jams and thicken yogurts and fillings.

In your body: Not digested; gut bacteria ferment it in the large intestine like other soluble fiber.

Found in: Apples, citrus peel, quince, plums, berries.

Gums and their sources
ThickenerE-numberSource
AgarE406red seaweed
CarrageenanE407red seaweed
Locust bean gumE410carob seeds
Guar gumE412guar beans
Xanthan gumE415made by bacteria fermenting sugar
Cellulose gum (CMC)E466chemically modified plant cellulose
Emulsifiers

A monoglyceride, E471

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  1. 1A water-loving head: glycerol with two free OH groups…
  2. 2…and a fat-loving tail: one fatty acid instead of a fat’s three. The head sits in water and the tail in oil, so droplets of one stay spread through the other.

What it is: Mono- and diglycerides (E471) keep bread soft and stop spreads and ice cream from separating. Lecithin (E322), from soy, sunflower or egg yolk, works the same way; egg yolk lecithin is what holds mayonnaise together.

In your body: Monoglycerides are digested like ordinary fat; your gut makes them itself when it digests a meal’s fat.

Too much: Early research in mice suggests some emulsifiers, such as cellulose gum (E466) and polysorbate 80 (E433), may disturb gut bacteria; evidence in people is limited.

Found in: Egg yolk, soybeans, sunflower seeds (lecithin).

carbon hydrogen oxygen · every molecule on this page is drawn at the same scale with every hydrogen shown · tap one to enlarge it

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Figure 7

Leavening, Anti-Caking and Flavor Enhancers

Baking soda, silica and MSG.

Acidity regulators

Bicarbonate, HCO₃⁻

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  1. 1Baking soda is sodium bicarbonate (E500). With an acid it gives off carbon dioxide gas, which makes batter rise.

What it is: A leavening agent and acidity regulator. Baking powder is baking soda already mixed with a dry acid.

In your body: Your pancreas releases bicarbonate to neutralize stomach acid as food enters the small intestine.

Found in: Made from mineral sources; buttermilk and yogurt supply the acid in many recipes.

Anti-caking agents
Keeping powders flowingSilicon dioxide (silica, E551) and calcium silicate (E552) soak up moisture so salt, spice mixes and powdered drinks do not clump. Silica is the same compound as quartz sand, in a fine powder.
Flavor enhancers

Monosodium glutamate, MSG, E621

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GlutamateC₅H₈NO₄⁻

SodiumNa⁺

  1. 1Glutamate is the amino acid glutamic acid (see Protein)…
  2. 2…with one acid group charged. In water MSG splits into glutamate and a sodium ion.

What it is: A flavor enhancer that tastes savory, or umami. It was first isolated from kombu seaweed in Japan in 1908.

In your body: Your body handles glutamate from MSG the same way as glutamate from tomatoes or cheese; most is used for energy by the gut itself.

Too much: The FDA classes it as GRAS and requires it to be listed by name. Blinded studies have not consistently reproduced the symptoms some people report. MSG has about a third of the sodium of table salt.

Found in: Free glutamate is high in tomatoes, parmesan, mushrooms, soy sauce, kombu and anchovies. Inosinate (E631) and guanylate (E627), from meat, fish and mushrooms, boost umami further.

carbon hydrogen oxygen nitrogen sodium · every molecule on this page is drawn at the same scale with every hydrogen shown · tap one to enlarge it

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Figure 8

Sweeteners

Intense sweeteners, sugar alcohols and the ADI.

Sweeteners

Aspartame, E951, C₁₄H₁₈N₂O₅

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  1. 1Aspartic acid…
  2. 2…joined to phenylalanine, two ordinary amino acids…
  3. 3…with a methyl ester cap that is released as methanol, in amounts smaller than from fruit juice.

What it is: About 200 times sweeter than sugar, used in diet drinks, yogurt and tabletop sweeteners. It breaks down with heat, so it is not used for baking.

In your body: Digested into its two amino acids and methanol. People with phenylketonuria (PKU) cannot break down phenylalanine, so labels must warn “Phenylketonurics: contains phenylalanine.”

Too much: In 2023 the WHO’s cancer agency classed it as “possibly carcinogenic” (the lowest level, based on limited evidence), while the WHO/FAO expert committee kept its acceptable daily intake at 40 mg per kg of body weight. The FDA’s is 50 mg per kg.

Found in: Not in whole foods.

Sucrose and sucralose, E955

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Sucrosetable sugar

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SucraloseE955

  1. 1Three OH groups in sugar…
  2. 2…are swapped for chlorine. The result is about 600 times sweeter, heat-stable, and mostly passes through without being absorbed.

Steviol glycosides (E960), from the leaves of the stevia plant, are 200–300 times sweeter than sugar.

Erythritol, a sugar alcohol, E968

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  1. 1A short chain with an OH on every carbon: a sugar alcohol, or polyol.

What it is: About 60–70% as sweet as sugar with almost no calories: it is absorbed and passed unchanged in urine.

Too much: A 2023 observational study linked high blood erythritol to heart attack and stroke; it does not show cause. Other polyols, such as sorbitol (E420) and xylitol (E967), cause gas and diarrhea in large amounts. Xylitol is poisonous to dogs.

Found in: Small amounts in pears, melons, grapes, mushrooms and fermented foods.

Acceptable daily intake
ADIThe amount per kilogram of body weight that can be eaten every day for a lifetime without appreciable risk, usually set at one hundredth of the highest dose that caused no harm in animal studies. A 70 kg adult would need roughly 9 to 14 cans of diet soda a day to reach aspartame’s ADI.

carbon hydrogen oxygen nitrogen chlorine · every molecule on this page is drawn at the same scale with every hydrogen shown · tap one to enlarge it

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Figure 9

Where additives are used in the body

Tap a number on the cell to see what that part does; the list below it gives every other place in the body.

Most additives do their job in the food; in you, they are absorbed and cleared, or pass through to the gut bacteria.

Inside a cell

A typical animal cell, not to scale. Real cells hold thousands of ribosomes and hundreds of mitochondria.

Most additives never reach your cells intact; a few do, and here is where.

  1. Emulsifiers like lecithin are digested like normal fats; polysorbate 80 and carboxymethylcellulose mostly act in the gut, where animal studies show they can thin the mucus layer.
  2. Citric acid (E330) is the same citrate the cell makes in its energy cycle.
  3. Ascorbic acid (E300) and tocopherols (E306) are vitamins C and E, acting as antioxidants as they do in food.
  4. Nitrite from cured meat can form nitrosamines, which can damage DNA; ascorbate added to cured meat cuts this.
  5. Liver enzymes here break down many synthetic additives such as colors and sweeteners so they can be removed.
  6. Some particles, like titanium dioxide (E171, banned in the EU since 2022), can be taken up into these sacs.

Everywhere else in the body

Each item, the places outside the cell where it is found or used, and what it does there.

Sweeteners

  • Tongue. Fit the sweet receptor; many are hundreds of times sweeter than sugar.
  • Small intestine. Aspartame is digested to phenylalanine, aspartic acid and a little methanol, which is why labels warn people with PKU.
  • Large intestine. Sucralose mostly passes through; bacteria strip stevia’s sugars off before it is absorbed.
  • Liver. Clears absorbed steviol.
  • Kidneys. Sucralose, acesulfame K and saccharin that are absorbed leave mostly unchanged in urine.

Sugar alcohols

  • Teeth. Mouth bacteria can’t ferment them well, so they don’t cause cavities.
  • Small intestine. Absorbed only partly.
  • Large intestine. Bacteria ferment the rest: gas, and loose stools in large amounts.
  • Kidneys. Erythritol is mostly absorbed and leaves in urine.

MSG and flavor enhancers

  • Tongue. Glutamate fits the umami receptor.
  • Small intestine. Its cells burn most of the glutamate you eat as their own fuel.

Preservatives

  • Stomach. Nitrite can react with amines to form nitrosamines, which is why vitamin C is added to cured meat.
  • Blood. Very large amounts of nitrite turn hemoglobin into a form that can’t carry oxygen; babies are most at risk.
  • Liver. Joins benzoate to glycine; sorbate and propionate are burned like fats.
  • Kidneys. Pass the benzoate product (hippuric acid) out in urine.
  • Airways. Sulfites can trigger asthma in sensitive people.

Acids and antioxidants

  • Teeth. Citric and phosphoric acid in drinks can wear enamel.
  • Stomach. Food acids simply add to stomach acid.
  • Every tissue. Ascorbic acid and tocopherols work as vitamins C and E.

Colors

  • Small intestine. Many synthetic colors are poorly absorbed.
  • Large intestine. Bacteria split azo dyes into smaller parts.
  • Liver. Clears absorbed colors into bile or urine.
  • Brain and behavior. A UK study linked some colors to more hyperactivity in some children, so the EU requires a warning.

Thickeners and emulsifiers

  • Large intestine. Gums are fermented by bacteria, like fiber.
  • Gut lining. In mice, the emulsifiers carboxymethylcellulose and polysorbate 80 thinned the mucus layer; human evidence is early.

Phosphates and minerals

  • Small intestine. Absorbs phosphate additives more fully than the phosphorus in whole foods.
  • Kidneys. Clear the extra; people with kidney disease are told to limit them.
  • Bones. Phosphorus is balanced with calcium in bone.
  • Gut immune patches. Some tiny titanium dioxide particles are taken up there; most pass through.
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Figure 10

Putting It All Together

The whole lesson in one table, plus a few extras.

The E-number ranges
RangeMain jobExamplesAlso found in whole foods
E100scolorscurcumin E100, Red 40 E129, titanium dioxide E171 (banned in the EU)turmeric, beets (E162), paprika (E160c)
E200spreservativessorbates E200–E203, benzoates E210–E213, sulfites E220–E228, nitrite E250rowan berries, cranberries, vegetables (nitrate)
E300santioxidants and acidsvitamin C E300, tocopherols E306, citric acid E330, lecithin E322, BHA E320, BHT E321citrus, nuts and seeds, egg yolk
E400sthickeners, gels and emulsifierspectin E440, guar E412, xanthan E415, carrageenan E407, mono- and diglycerides E471apples, citrus peel, seaweed, beans
E500sacidity regulators and anti-caking agentsbaking soda E500, silica E551—
E600sflavor enhancersMSG E621, guanylate E627, inosinate E631tomatoes, parmesan, mushrooms, kombu
E900ssweeteners and glazesaspartame E951, sucralose E955, steviol glycosides E960, erythritol E968, beeswax E901, carnauba wax E903stevia leaves, pears and grapes, honeycomb
Worth knowing

An E-number is an approval, not a warning

It only means the additive passed an EU safety review; many are compounds also found in whole foods, such as vitamin C (E300) and pectin (E440).

“Uncured” is still cured

Meats labeled “uncured” or “no nitrites added” are usually cured with celery powder, whose natural nitrate bacteria turn into nitrite.

Additives cluster in ultra-processed foods

Whole foods need almost none; a long list of additives is one sign that a food is highly processed.

General education, not medical advice. Facts follow regulators and mainstream sources (US FDA, European Food Safety Authority, WHO/FAO JECFA, standard food-science texts).

Quiz

Tap a question to see the answer

1. What does an E-number tell you?

That the additive has passed an EU safety review; the hundreds digit roughly gives its job (Figure 1).

2. How are additives shown on a US label?

By name, such as “sodium benzoate,” not by E-number (Figure 2).

3. Where does most added citric acid come from?

Fermenting sugar with a mold, Aspergillus niger (Figure 2).

4. Which additive did the EU ban in 2022, and why?

Titanium dioxide, E171, because harm to DNA could not be ruled out (Figure 3).

5. What does the E200 range do?

Preserves food against microbes (Figure 4).

6. Why is sodium nitrite (E250) in cured meat?

It stops the botulism bacterium and gives the pink color and cured flavor (Figure 4).

7. Who needs to watch for sulfites?

People with sulfite-sensitive asthma; US labels must declare 10 ppm or more (Figure 4).

8. What do BHA and BHT do?

They stop fats from going rancid (Figure 5).

9. What is pectin made of, and where does it come from?

Chains of galacturonic acid, from apples and citrus peel (Figure 6).

10. How does an emulsifier keep oil and water mixed?

It has a water-loving head and a fat-loving tail (Figure 6).

11. What is E621, and which whole foods are rich in the same compound?

Monosodium glutamate; tomatoes, parmesan, mushrooms, soy sauce, kombu (Figure 7).

12. How does sucralose differ from sugar?

Three OH groups of sucrose are replaced by chlorine, making it about 600 times sweeter (Figure 8).

Next lesson: Module 3 · Antinutrients →