LeClaire Institute

Module 3 · Flavorings

Flavor is taste plus smell. This lesson covers the five basic tastes and the receptors that sense them, the umami molecules glutamate and inosinate, four common aroma molecules (vanillin, cinnamaldehyde, menthol and limonene), and what natural and artificial flavor mean on a label. Each molecule is shown in ball-and-stick form with what it does, the cautions, and where it is found. The figure is on the left and the lesson script on the right; the quiz is at the end.

Previous lesson: Module 3 · Bioactives

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Figure 1

The Flavorings Family Tree

Flavor is taste plus smell: five tastes, many aromas, and the label lines.

What the tree shows
Taste plus smellWhat we call flavor is mostly two senses working together: a handful of tastes on the tongue and thousands of aromas sensed in the nose. Pinch your nose and a jelly bean tastes only sweet; the cherry or lemon comes back when you let go.
Molecules meet receptorsEvery taste and smell starts when a molecule fits a receptor protein on a sensory cell. This lesson shows the molecules and the receptors they fit.
Flavorings on the labelMost added flavorings are aroma molecules, listed as “natural flavor” or “artificial flavor”; a few, such as MSG, act on taste. Figures 7 and 8 explain what those label lines do and do not tell you.
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Figure 2

How Flavor Works

The five basic tastes, their receptors, and why smell does most of the work.

The five basic tastes
TasteWhat it signalsReceptorExamples
Sweetenergy (sugars)one receptor made of two parts, T1R2 + T1R3sugar, fruit, honey, sweeteners
Umamiprotein (amino acids)T1R1 + T1R3tomato, cheese, broth, soy sauce
Saltysodium, a needed mineralthe sodium channel ENaC, plus others at high saltsalt, soy sauce, olives
Souracid: unripe or spoiled foodOTOP1, a channel that lets hydrogen ions inlemon, vinegar, yogurt
Bitterpossible poisonabout 25 different TAS2R receptorscoffee, dark greens, hops, tonic water
Smell does most of the work
Through the back of the mouthAs you chew, aroma molecules rise from the back of the mouth into the nose. Humans have about 400 kinds of smell receptor, and each aroma molecule switches on a pattern of them, so we can tell apart a huge number of smells.
The tongue map is a mythEvery part of the tongue that has taste buds can sense all five tastes. Each taste bud holds 50 to 100 receptor cells, and they are replaced about every week or two.
Hot, cool and tinglingChili heat, mint cool and the fizz of carbonation are not tastes or smells but touch and pain nerves reacting to chemicals (see Bioactives, Figure 7).
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Figure 3

Sweet and Bitter

One receptor for sweet, about 25 for bitter.

Sweet

Sweet: a sugar fits the sweet receptor

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  1. 1Table sugar, sucrose: glucose and fructose joined through one oxygen (see Carbohydrates). Its OH groups grip the sweet receptor’s clamp-like pocket.

What it is: Sweet taste is sensed by one receptor built from two proteins, T1R2 and T1R3. Sugars, sugar alcohols, artificial sweeteners and even a few plant proteins all fit it, in different spots.

In your body: Sweetness signals energy, and babies prefer it from birth. The same receptor sits in the gut, where it helps control sugar absorption and hormone release. Sweeteners such as sucralose fit it hundreds of times more tightly than sugar, so a tiny amount tastes as sweet (see Additives).

Too much: Liking for sweetness falls when people eat less sugar for a few weeks. The WHO advises keeping free sugars below 10% of calories.

Found in: Sugars in fruit, honey, milk and added sugar; sweeteners such as sucralose, aspartame, stevia and monk fruit.

Bitter

Bitter: many receptors for many molecules

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  1. 2Caffeine (see Bioactives) fits several bitter receptors. Bitter compounds share no single shape, which is why we need about 25 kinds of receptor to catch them.

What it is: Bitter taste is sensed by about 25 different TAS2R receptors, each tuned to a range of molecules. It is the most sensitive taste, because many plant poisons are bitter.

In your body: Bitterness warns us, and it can trigger gagging at high levels. People differ genetically: about a quarter of people are strong tasters of certain bitter compounds, through a variant of the TAS2R38 receptor, and often find broccoli, kale and grapefruit harsher.

Too much: Bitterness is often a sign of something valuable as well, since many polyphenols are bitter; cooking, salt, fat and sweetness all soften it.

Found in: Coffee, tea, cocoa, beer hops, dark leafy greens and brassicas, grapefruit (naringin), olives, tonic water (quinine).

carbon hydrogen oxygen nitrogen · every molecule on this page is drawn at the same scale with every hydrogen shown · tap one to enlarge it

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Figure 4

Sour and Salty

Tastes made by ions: hydrogen and sodium.

Sour

Sour: hydrogen ions

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HydroniumH₃O⁺

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Citric acidC₆H₈O₇

  1. 1Acids release hydrogen ions, which in water ride on a water molecule as hydronium. Sour cells sense them through a channel called OTOP1 that lets them in.
  2. 2Citric acid, the sour of lemons, has three acid groups, each able to release a hydrogen ion.

What it is: Sour is the taste of acid. It is detected by hydrogen ions flowing into sour-sensing cells through the OTOP1 channel.

In your body: Sourness warns of unripe fruit and spoiled food, yet in moderation it is refreshing. Acids such as citric, lactic, malic and acetic acid are added to foods for tartness and to preserve them (see Additives).

Too much: Frequent acidic drinks, including sodas, juices and sports drinks, can erode tooth enamel; rinsing with water helps.

Found in: Lemons, limes and other citrus (citric acid), apples (malic), grapes (tartaric), yogurt and sauerkraut (lactic), vinegar (acetic), sodas (phosphoric and citric).

Salty

Salty: sodium ions

SodiumNa⁺

ChlorideCl⁻

PotassiumK⁺

What it is: Salt taste is mainly the taste of sodium ions. At normal levels they enter salt cells through a sodium channel called ENaC; very high salt also switches on bitter and sour cells, which is why heavily salted food tastes harsh.

In your body: Sodium is needed for nerves, muscles and fluid balance (see Major Minerals), and salt taste helps us seek it. Most people eat far more than they need. Potassium chloride, used in salt substitutes, tastes salty but also bitter and metallic, because potassium does not fit the sodium channel as well.

Too much: The FDA Daily Value for sodium is 2,300 mg, about one teaspoon of salt. Taste for salt adapts: after a few weeks of eating less, lightly salted food tastes right.

Found in: Table salt, and above all processed and restaurant food: bread, cured meats, cheese, soups, sauces and snacks supply most dietary sodium.

carbon hydrogen oxygen sodium chlorine potassium · every molecule and ion on this page is drawn at the same scale with every hydrogen shown · tap one to enlarge it

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Figure 5

Umami and Glutamate

The savory taste, and the facts about MSG.

Umami

Glutamate and inosinate

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GlutamateC₅H₈NO₄⁻

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Inosinate (IMP)C₁₀H₁₃N₄O₈P

  1. 1Glutamate is one of the amino acids in every protein (see Protein). Free, unattached glutamate is what tastes savory…
  2. 2…and this second acid group is what the umami receptor grips. MSG is just glutamate paired with a sodium ion.
  3. 3Inosinate, from meat and fish, binds the same receptor at a different spot and multiplies glutamate’s effect.

What it is: Umami is the savory taste of free glutamate, sensed by the T1R1 + T1R3 receptor. The Japanese chemist Kikunae Ikeda named it in 1908 after finding glutamate in kombu seaweed broth, and he went on to make monosodium glutamate (MSG, E621).

In your body: Umami signals protein. Inosinate (E631) and guanylate (E627) strongly boost it, which is why dashi made from kombu and bonito flakes, or tomato with parmesan, tastes so rich. Your body handles glutamate from MSG the same way as glutamate from tomatoes or cheese; most is used as fuel by the gut itself.

Too much: The US FDA lists MSG as generally recognized as safe, and controlled studies have not consistently reproduced the headaches and flushing some people attribute to it; a few may react to large doses on an empty stomach. MSG has about a third as much sodium as table salt, so it can help cut sodium.

Found in: Naturally high in parmesan and aged cheeses, tomatoes, mushrooms, soy sauce, miso, fish sauce, kombu, cured ham, yeast extract and broths. On labels: monosodium glutamate, yeast extract, hydrolyzed protein, disodium inosinate and guanylate.

carbon hydrogen oxygen nitrogen phosphorus · every molecule on this page is drawn at the same scale with every hydrogen shown · tap one to enlarge it

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Figure 6

Four Aroma Molecules

Vanillin, cinnamaldehyde, menthol and limonene.

Four aroma molecules

Vanillin

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  1. 1An aldehyde group (C=O with H) on a ring…
  2. 2…plus the methoxy and OH pair of guaiacol, the smoke molecule in Processing. Vanillin is the main flavor of cured vanilla beans.

What it is: The most widely used flavor molecule in the world. Vanilla beans develop it during weeks of curing, but less than 1% of the vanillin used comes from vanilla beans; the rest is made from other sources (Figure 7).

In your body: No nutritional role; used at tiny levels. Real vanilla contains hundreds of other aroma compounds, which is why it tastes rounder than pure vanillin.

Found in: Vanilla beans and extract, chocolate, ice cream, baked goods, soft drinks; small amounts form naturally in oak-aged wine and whisky.

Cinnamaldehyde

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  1. 3A ring, a double bond and an aldehyde. Besides its smell, cinnamaldehyde switches on TRPA1, the nerve receptor behind cinnamon’s warm sting.

What it is: The main aroma compound of cinnamon bark, making up most of its essential oil.

In your body: Food amounts are safe. Cinnamon supplements for blood sugar have shown small and inconsistent effects.

Too much: Cassia, the common cinnamon in North America, also contains coumarin, which can harm the liver in large amounts; EFSA set a tolerable intake of 0.1 mg per kg of body weight a day. Ceylon cinnamon has very little. Inhaling dry ground cinnamon can damage the lungs.

Found in: Cinnamon (cassia and Ceylon), cinnamon gum and candy, baked goods, colas, chai.

Menthol

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  1. 4A carbon ring with one OH. Menthol switches on TRPM8, the receptor that senses cold, so mint feels cool at room temperature.

What it is: The cooling compound of peppermint and corn mint.

In your body: Enteric-coated peppermint oil can ease symptoms of irritable bowel syndrome in some studies. Menthol is also used in cough drops and rubs for its cool feeling.

Too much: Concentrated peppermint oil can worsen heartburn and is toxic if swallowed in large amounts; it should not be put on the faces of young children.

Found in: Peppermint, mint tea, gum, candy, toothpaste, cough drops. Spearmint gets its flavor mainly from a different molecule, carvone.

Limonene

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  1. 5Ten carbons and only hydrogens: a terpene, built from two isoprene units (see Bioactives, Figure 4). Its mirror-image twins smell different: one of oranges, one more of pine.

What it is: The main compound of citrus peel oil, released when you peel or zest an orange.

In your body: Food amounts are safe and it is generally recognized as safe by the US FDA. It is also widely used in cleaners and fragrances.

Too much: Concentrated limonene can irritate skin, and it reacts with ozone in indoor air to form other compounds.

Found in: Orange, lemon, lime and grapefruit peel and zest, marmalade, citrus sodas and candies, dill, caraway.

carbon hydrogen oxygen · every molecule on this page is drawn at the same scale with every hydrogen shown · tap one to enlarge it

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Figure 7

Natural vs Artificial Flavors

Identical molecules from different sources, and what the words mean.

Natural vs artificial

Same molecule, different label

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Ferulic acidC₁₀H₁₀O₄ · from rice bran

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GuaiacolC₇H₈O₂ · from petroleum

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Ethyl vanillinC₉H₁₀O₃ · made only by chemists

  1. 1Microbes can trim ferulic acid’s three-carbon chain down to vanillin’s aldehyde. Because it starts from a plant and uses fermentation, the result can be called natural.
  2. 2Most vanillin is built from guaiacol made from petroleum. The vanillin is identical, molecule for molecule, but it must be called artificial.
  3. 3Ethyl vanillin has one extra carbon, does not occur in vanilla, and tastes two to four times stronger. It is always an artificial flavor.

What it is: In US rules (21 CFR 101.22), a natural flavor is one whose flavor compounds come from a plant or animal source, such as a spice, fruit, vegetable, meat or fermentation; an artificial flavor is any flavoring substance not from those sources. EU rules (Regulation 1334/2008) draw a similar line.

In your body: Natural and artificial versions of the same molecule act the same in the body. “Natural” describes the source, not the nutrition or the safety; both kinds are used in tiny amounts and are reviewed for safety, in the US often through an industry expert panel (FEMA GRAS) and in the EU by EFSA.

Too much: A “natural flavor” can be a complex mixture of dozens of compounds plus solvents and carriers, and the recipe does not have to be disclosed, although major allergens must be.

Found in: Natural or artificial flavors are in most packaged drinks, snacks, cereals, yogurts, candy and sauces.

carbon hydrogen oxygen · every molecule on this page is drawn at the same scale with every hydrogen shown · tap one to enlarge it

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Figure 8

Reading Flavor Lines on a Label

What each flavor-related ingredient line tells you.

Reading the flavor lines
Label lineWhat it means
Natural flavor(s)flavor compounds from a plant, animal or fermentation source; may be one compound or a blend
Artificial flavor(s)flavor compounds made by chemical synthesis; must be stated, and the front label must say “artificially flavored” when it supplies the main flavor
Natural and artificial flavorsa mix of both
Vanilla extractby US standard, vanilla beans extracted in at least 35% alcohol at a set strength
Vanillinartificial unless stated as natural; ethyl vanillin is always artificial
Spicesmay be listed as a group without naming each one; paprika, turmeric and saffron add color, so they must also be declared as coloring
Monosodium glutamate, disodium inosinate, guanylateumami flavor enhancers; MSG must be named
Yeast extract, hydrolyzed proteinnatural sources of free glutamate; a food containing them cannot claim “No MSG”
Smoke flavoringfiltered smoke condensate (see Processing, Figure 4)
On the front of the pack
“Strawberry yogurt” vs “strawberry flavored”A name like “strawberry yogurt” suggests the fruit is there. “Strawberry flavored” or “naturally flavored” can mean the taste comes mostly or entirely from flavorings. Check the ingredient list for the fruit itself.
Natural vanilla in the EUIn the EU, “natural vanilla flavouring” must get at least 95% of its flavor from vanilla.
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Figure 9

Where flavorings are used in the body

Tap a number on the cell to see what that part does; the list below it gives every other place in the body.

Flavor is built in the mouth, the nose and the brain; then the molecules are digested like any other food.

Inside a cell

A typical animal cell, not to scale. Real cells hold thousands of ribosomes and hundreds of mitochondria.

Taste and smell are cell-membrane events.

  1. Sweet, umami and bitter molecules bind receptor proteins (T1R, T2R families); salt and sour act through ion channels.
  2. Aroma molecules bind odorant receptors on nose cells; people have about 400 kinds.
  3. Capsaicin from chili opens the TRPV1 channel, the same one heat opens; menthol opens TRPM8, the cold channel.
  4. Inside taste cells, the signal is passed on by calcium released from the ER, then the cell signals the nerve.
  5. Liver enzymes break down most flavor compounds so they can be removed.
  6. Genes for taste receptors vary, which is why some people find cilantro soapy or bitter vegetables extra bitter.

Everywhere else in the body

Each item, the places outside the cell where it is found or used, and what it does there.

Taste molecules

  • Tongue. Taste buds sense sweet, bitter, sour, salty and umami.
  • Soft palate and throat. Also have taste buds.
  • Gut. Has sweet and bitter receptors that help release gut hormones.
  • Airways. Bitter receptors there help sweep out mucus.
  • Brain. The taste area (in the insula) receives the signals.

Aroma molecules

  • Nose. About 400 kinds of smell receptor in the roof of the nose.
  • Mouth to nose. Aromas rise from the back of the mouth as you chew, which is most of flavor.
  • Brain. Smell goes straight to areas for emotion and memory.

Feel: heat, cold, bite and fizz

  • Mouth, nose and eyes. The trigeminal nerve feels chili (TRPV1), menthol’s cool (TRPM8), mustard and wasabi (TRPA1) and the fizz of carbonation.
  • Eyes. An onion compound reaches them and makes them water.

Flavor in the brain

  • Brain. Combines taste, smell, texture and heat into one flavor; that’s why food tastes flat with a blocked nose.

Digesting and clearing them

  • Stomach and small intestine. Absorb flavor molecules like the rest of the food.
  • Liver. Breaks most of them down; vanillin becomes vanillic acid.
  • Kidneys. Send the products out in urine; asparagus gives urine its smell this way.
  • Lungs and skin. Garlic compounds leave in the breath and sweat.
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Figure 10

Putting It All Together

The whole lesson in one table, plus a few extras.

Flavor at a glance
Sense or flavoringMoleculeReceptorMain sourcesGood to know
Sweetsucrose and other sugars, sweetenersT1R2 + T1R3fruit, honey, added sugarliking falls when you eat less
Bittercaffeine, quinine, polyphenolsabout 25 TAS2Rscoffee, greens, hops, grapefruitstrong tasters vary genetically
Sourhydrogen ions from acids such as citric acidOTOP1 channelcitrus, vinegar, yogurtacid drinks erode enamel
Saltysodium ionsENaC and otherssalt, processed foodDaily Value 2,300 mg sodium
Umamiglutamate, inosinate, guanylateT1R1 + T1R3cheese, tomato, soy sauce, brothMSG has a third of salt’s sodium
Aromavanillin, cinnamaldehyde, menthol, limoneneabout 400 smell receptorsvanilla, cinnamon, mint, citrusmost of what we call flavor
Flavor labelsnatural or artificial versions of aroma molecules—most packaged food“natural” is about the source, not safety
Worth knowing

Losing smell

Colds, COVID-19, head injuries, some medicines and aging can dull smell, which makes food seem tasteless even though taste itself works. People with smell loss often add more salt and sugar; herbs, spices, acidity and texture can help instead.

Kokumi

Some researchers propose a sixth sensation, kokumi, a richness and mouthfulness from certain small peptides sensed by the calcium-sensing receptor. Fat taste is another candidate under study.

Sensory-specific satiety

The pleasure of one flavor fades as you eat it, while a new flavor still appeals, which is one reason there is always room for dessert, and why very varied buffets lead people to eat more.

General education, not medical advice. Facts follow mainstream sources (US FDA, EFSA and EU regulations, NIH, standard food-science and sensory-science texts).

Quiz

Tap a question to see the answer

1. Which two senses make up most of what we call flavor?

Taste on the tongue and smell in the nose (Figure 1).

2. Name the five basic tastes.

Sweet, sour, salty, bitter and umami (Figure 2).

3. Is the tongue map, with sweet at the tip and bitter at the back, correct?

No; every area with taste buds senses all five tastes (Figure 2).

4. How many receptors sense sweet, and how many sense bitter?

One sweet receptor (T1R2 + T1R3) and about 25 bitter receptors (Figure 3).

5. Why do some people find broccoli and grapefruit very bitter?

They carry a receptor variant (TAS2R38) that makes them strong bitter tasters (Figure 3).

6. What do sour cells actually detect?

Hydrogen ions released by acids, entering through the OTOP1 channel (Figure 4).

7. Why do potassium chloride salt substitutes taste bitter?

Potassium does not fit the sodium channel well and also activates bitter cells (Figure 4).

8. What molecule causes umami taste, and what boosts it?

Free glutamate; inosinate and guanylate boost it strongly (Figure 5).

9. How does the sodium in MSG compare with table salt?

MSG has about a third as much sodium (Figure 5).

10. Which receptor does menthol switch on, and what does it normally sense?

TRPM8, the cold receptor (Figure 6).

11. Can the same vanillin molecule be natural or artificial?

Yes; the label depends on the source: from a plant via fermentation it can be natural, from petroleum guaiacol it is artificial (Figure 7).

12. Can a food containing yeast extract claim “No MSG”?

No; yeast extract is a natural source of free glutamate (Figure 8).

Next lesson: Module 4 · Final Review →